Here, we report that Suppressor of fused, Su(fu), causes the Rdx switch. Transient effector-reporter assays for functional analysis of transcription factors and detection of protein-protein interactions using the trans-repressive activity of SRDX repression domain are also described. formation by regulating transcription factor Cubitus interruptus (Ci). Strategies for construction of the chimeric repressors and their expression in transgenic plants are described. SMXL6 represses transcription by interacting with TPL/TPR proteins in a manner that depends on its ethylene-responsive-element-binding-factor-associated amphiphilic repression (EAR) motif 7,8,9,10. VP16 is an acidic protein derived from herpes simplex virus that exhibits strong transactivation activity in a wide range of species, including plants (Sadowski et al., 1988 Liang et al. This system is simple and effective and can be used as a powerful tool not only for functional analysis of redundant transcription factors but also for the manipulation of plant traits by active suppression of the gene expression. repression domain (SRDX) in the rice Taichung 65 back- ground. As a result, the transgenic plants that express a chimeric repressor exhibit phenotypes similar to loss-of-function of the alleles of the gene encoding the transcription factor. Soybean (Glycine max), as an important oilseed crop, is constantly threatened by abiotic stress, including that caused by salinity and drought. In this system, a chimeric repressor that is produced by fusion of a transcription factor to the plant-specific EAR-motif repression domain (SRDX) suppresses target genes of a transcription factor dominantly over the activity of endogenous and functionally redundant transcription factors. By contrast, the average phenolic acid yield in the SmMYB1-SRDX lines was 34.91. ![]() (A) The phenotypes of 60-day-old WT and OsNTL5C transgenic plants. Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1. FIGURE 1 Phenotypic and molecular characterization of the OsNTL5C, OsNTL5C-SRDX, and OsNTL5C-VP transgenic lines of rice. Chimeric REpressor gene Silencing Technology (CRES-T) is a useful tool for functional analysis of plant transcription factors. The application of sequencespecific transcriptional repression tools can be used to assess functional genomics in detail and other related biotechnological applications. Hence, the results of phenotypic analysis of these transgenic lines confirm that OsNTL5 regulates flowering in rice as a transcriptional repressor.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |